BnSIP1-1 Involves in Light Response and Regulation of Endogenous Hormones and Flowering Time of Brassica Napus

Junling Luo,Xiaohong Yan,Xinhua Zeng,Weihua Jiang,Gang Wu,Fengling Mei,Shaohua Tang

doi:10.1007/s00344-020-10250-w

Abstract

BnSIP1-1 is a trihelix transcription factor family gene which functions in abiotic stress response and abscisic acid (ABA) signaling during seed germination and seedling growth of Brassica napus. In the present study, further sequence analysis and phenotype identification indicated that this gene had roles in light regulation and flowering of reproductive growth stage. Many phytohormones responsive cis-acting elements, including TC-rich repeats, GARE-motif, and TCA and TGA elements, were identified in the promoter sequence of BnSIP1-1. The expression of BnSIP1-1 was regulated by light period and remarkable higher expression level of BnSIP1-1 was detected in roots than in leaves. Overexpression of BnSIP1-1 in Arabidopsis delayed flowering time for 3–5 days in transgenic plants. In addition, we also found BnSIP1-1 can respond to abiotic and ABA stress (treated with 200 mM NaCl, 300 mM mannitol or 50 μM ABA for 0, 1, 6, and 24 h) in B. napus through adjusting not only ABA but also other endogenous hormones, including indole-3-acetic acid and salicylic acid. Moreover, jasmonates (JA) signaling pathway was found not involving in the pathway of BnSIP1-1 responding to abiotic stresses.

Highlights

Brassica napus 6b INTERACTING PROTEIN1-1, BnSIP11, a SIP subfamily gene of trihelix transcription factor family, was recently characterized playing important roles in abiotic stress response and abscisic acid (ABA) signaling based on the previous work of our lab (Luo et al 2017)
BnSIP1-1 was identified as a trihelix family gene mediating abiotic stress response and ABA signaling in B. napus (Luo et al 2017)
Overexpressing BnSIP1-1 could indicated that the value of a t test was p < 0.05 and p < 0.01, respectively, and a significant difference between the different transgenic lines and wildtype. b Morphological comparison of flowering time between BnSIP1-1 overexpressing transgenic plants and wildtype plants increase germination ability of seeds under drought, salt, and ABA treatment, as well as improving osmotic stress tolerance and ABA sensitivity during early stage of vegetative growth (Luo et al 2017)

Summary

Introduction

Brassica napus 6b INTERACTING PROTEIN1-1, BnSIP11, a SIP subfamily gene of trihelix transcription factor family, was recently characterized playing important roles in abiotic stress response and ABA signaling based on the previous work of our lab (Luo et al 2017). BnSIP1-1 was the first trihelix gene functionally identified in B. napus. The transcript level of BnSIP1-1 can be upregulated by drought, ionic toxicity, and ABA treatment. Overexpression of BnSIP1-1 could improve the germination rate of seeds under osmotic and salt stress as well as increasing the tolerance to osmotic stress during the seedling stage (Luo et al 2017). The family genes were first known as GT factors because

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Conclusion