Abstract
Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS3-silenced (BnSGS3-Si) oilseed rape (cv. Zhongshuang No. 6) plants. These three viruses are Oilseed rape mosaic virus (ORMV), Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation) for breeding virus-resistant oilseed rape varieties.
Highlights
Virus disease, next to white mold caused by Sclerotinia sclerotiorum, is one of the most important yield-limiting diseases of oilseed rape production in China [1]
Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV)), our results reveal that CMV accumulation was negatively correlated with expression levels of that BnSGS3 selectively inhibits accumulation, but promotes levels of BnSGS3, BnSGS3, suggesting whereas accumulations of both TuMV
The results showed that the amplification and R2 was greater than 0.99, indicating that there was a good linear relationship between Ct values efficiency (E) of Oilseed rape mosaic virus (ORMV), TuMV, and CMV viral plasmids was 86.2%, 88.7% and 84.9%, respectively, and the logarithm of the copy numbers and the standard curves were appropriate for calculation of and R2 was greater than 0.99, indicating that there was a good linear relationship between Ct values and virus quantity
Summary
Next to white mold caused by Sclerotinia sclerotiorum, is one of the most important yield-limiting diseases of oilseed rape production in China [1]. CMV can only partially inhibit PTGS and produces only a little amount of CMV virus RNA in Arabidopsis L1 plants, which is significantly lower than that in sgs mutants. As a result, these two viruses accumulated to high levels in the sgs mutants and L1 plants [4]. Using these transgenic plants upon inoculation with three different viruses (ORMV, TuMV and CMV), our results reveal that CMV accumulation was negatively correlated with expression levels of. TuMV and CMV), our results reveal that CMV accumulation was negatively correlated with expression levels of that BnSGS3 selectively inhibits accumulation, but promotes. Expression of BnSGS3, suggesting that BnSGS3 selectively inhibits CMV accumulation, but promotes TuMV and ORMV accumulation
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