BMP9‐induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/β‐catenin signalling

Abstract

Journal of Cellular and Molecular MedicineVolume 27, Issue 8 p. 1155-1156 CORRIGENDUMOpen Access BMP9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/β-catenin signalling This article corrects the following: BMP-9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/β-catenin signalling Ni Tang, Wen-Xin Song, Jinyong Luo, Xiaoji Luo, Jin Chen, Katie A. Sharff, Yang Bi, Bai-Cheng He, Jia-Yi Huang, Gao-Hui Zhu, Yu-Xi Su, Wei Jiang, Min Tang, Yun He, Yi Wang, Liang Chen, Guo-Wei Zuo, Jikun Shen, Xiaochuan Pan, Russell R. Reid, Hue H. Luu, Rex C. Haydon, Tong-Chuan He, Volume 13Issue 8bJournal of Cellular and Molecular Medicine pages: 2448-2464 First Published online: November 3, 2008 First published: 12 April 2023 https://doi.org/10.1111/jcmm.17715AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL In Tang et al.,1 the image for ‘BMP9+GFP Day 14’ was mistakenly duplicated with a higher magnification version of the image from ‘BMP9+FrzB Day 14’ in Figure 5E. The corrected figure is shown below. The authors confirm all results and conclusions of this article remain unchanged. FIGURE 5Open in figure viewerPowerPoint β-Catenin also plays an important role in BMP-9-induced late stage of osteogenic differentiation. (A) Synergistic effect between β-catenin and BMP-9 on osteocalcin promoter reporter. C3H10T1/2 cells were transfected with p6OSE2-Luc reporter and infected with varying titres of Adβ-Cat*, in the presence or absence of BMP-9 conditioned medium. At 48 h, cells were collected for luciferase assay. Data are present as mean ± SD. (B) Synergistic effect between β-catenin and Runx2 on osteocalcin promoter reporter. C3H10T1/2 cells were transfected with p6OSE2-Luc reporter and co-infected with varying titres of Adβ-Cat* and/or AdRunx2. At 48 h, cells were collected for luciferase assay. Data are present as mean ± SD. (C) Knockdown of β-catenin inhibits BMP-9-induced activation of osteocalcin promoter reporter. C3H10T1/2 cells were transfected with p6OSE2-Luc and infected with varying titres of AdR-simBC in the presence of BMP-9 conditioned medium. Cells were collected for luciferase at the indicated time-points. (D) Knockdown of β-catenin inhibits BMP-9-induced expression of late osteogenic markers. C3H10T1/2 cells were co-infected with AdBMP-9 and AdR-simBC or AdGFP for 10 days. Total RNA was isolated for RT-PCR and qPCR analysis using primers specific for mouse osteocalcin and osteopontin. Experiments were done in triplicate. (E) Silencing of β-catenin and FrzB overexpression inhibit BMP-9-induced mineralization. C3H10T1/2 cells were co-infected with AdBMP-9 and AdFrzB, AdR-simBC, or AdGFP. At 14 and 21 days after infection, cells were fixed and subjected to Alizarin Red S staining. Representative images are shown (magnification, 40×). REFERENCE 1Tang N, Song W-X, Luo J, et al. BMP-9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/beta-catenin signalling. J Cell Mol Med. 2009; 13(8B): 2448- 2464. doi:10.1111/j.1582-4934.2008.00569.x Volume27, Issue8April 2023Pages 1155-1156 FiguresReferencesRelatedInformation