BMP15 mRNA profile in granulosa cells from endometriosis patients undergoing in vitro fertilization

Abstract

Endometriosis is a female reproductive disorder that can cause infertility by lowering oocyte quality due to a low quality of oocytes. Granulosa cell assessment is a minimally invasive procedure for determining oocyte quality. Bone morphogenetic protein 15 (BMP15) also acts as a growth factor for oocyte derivatives that regulate proliferation and granulosa cell differentiation. In endometriosis, higher BMP15 expression is assumed to induce oocyte growth. This study aimed to determine the mRNA levels of BMP15 in granulosa cells from endometriosis patients undergoing in vitro fertilization. We analyzed samples from 12 patients with endometriosis and seven normal women using real-time PCR at Yasmin Clinic, Kencana, Cipto Mangunkusumo Hospital. Granulosa cells collected during oocyte retrieval were preserved using RNAlater® QIAGEN and were evaluated by absolute quantitative real-time PCR with pre-manufactured oligonucleotides as the standard. Results showed that although BMP15 did not significantly differ between the endometriosis patients and the controls (p > 0.05), the endometriosis patients had higher BMP15 mRNA levels than the controls. Therefore, further study is warranted to gain more comprehend data.