BMeS-p-A succinimidyl ester as a sulfonyl aniline dye labeling reagent

Abstract

Here, we describe the synthesis and properties of BMeS-p-A-NHS as a sulfonylaniline-based fluorescent labeling reagent. Introduction of a carboxyl group into the amino group of the sulfonylaniline skeleton was achieved by the ring-opening reaction of β-propiolactone. This small labeling reagent was water-soluble, photostable, solid-state emissive, and suitable for excitation with a versatile 405-nm laser diode, emitting green fluorescence with a large Stokes shift at 527 nm. The succinimidyl ester reactive group facilitated conjugation with substrates containing amino groups.