Bme585 I [5′-CCCGC(4/6)-3′], a new isoschizomer of restriction endonuclease Fau I, isolated from a strain of Bacillus mesentericus

Abstract

Bme585 I is a new member of the restriction endonuclease type IIS family. It was partially purified from the heterothrophic, mesophilic bacterial strain Bacillus mesentericus 585 by ammonium sulphate precipitation and phosphocellulose column chromatography. Bme585 I is a monomeric protein with a molecular mass of 62 kD. The enzyme is active over a broad pH range from 7.0 to 8.8, has a temperature optimum of 37°C and tolerance of NaCl in reaction buffer from 0 to 400 mM. Bme585 I recognizes the asymmetric sequence 5′-CCCGC(4/6)-3′ and is therefore an isoschizomer of restriction endonuclease Fau I.