Blue LED and succinic acid enhance the growth of Rhodobacter sphaeroides

Abstract

Rhodobacter sphaeroides is a purple non-sulfur photosynthetic bacteria that participates in the anoxic cycling of carbon both as the primary producer and as the light-stimulated consumers of the reduced organic compounds. In this study, six different organic acids, i.e. acetate, lactate, oxaloacetate, malate, succinate, and citrate, were selected and used to analyze the relationships between the organic acid source and the cell growth. The C4 compound exhibited an enhanced cell growth compared to the other organic acids, and the growth rate of R. sphaeroides that was grown with 0.03 M succinic acid was significantly 3.2-fold faster than the C6 compound of 0.03 M citrate. Additionally, the cell growth of R. sphaeroides was enhanced with increasing light intensity, and the growth rate and the dry cell weight of R.sphaeroides that were grown under the light conditions of 15 W/m2 were 2.0- and 1.2-fold higher than R. sphaeroides at 3 W/m2. Therefore, the high light intensity probably affected the growth of R. sphaeroides. Moreover, the blue-colored light emitting diode (LED) exhibited a highest growth rate and cell concentration of R. sphaeroides among the various types of LEDs, and the enhanced cell growth phenomenon under the blue LED conditions was dramatically stimulated at low concentrations of succinic acid, which was compensatory for succinic acid. Therefore, a high light intensity and a blue LED as the light source were necessary for the enhanced cell growth for the C4 organic acid, i.e. succinic acid.