Blood-compatibility of polyurethane/liquid crystal composite membranes.

Abstract

Polyurethane/liquid crystal composite membranes were first suggested to be used as biomaterials. In our work, three series of polyurethane/liquid crystal composite membranes based on three different kinds of liquid crystal compounds [N-(-4-methyoxybenzylidene)-4'-heptylaniline, 4-pentyl-4'-nitrile-biphenyl and cholesteryl oleyl carbonate] were prepared by casting on glass plates from a tetrahydrofuran (THF) solution of polymer and liquid crystal at room temperature. In our opinion, the formation of liquid crystal phase on the composite membrane surface is the basic requirement for getting better biomaterial. The result of this work is in accordance with our opinion. The effect of liquid crystal content on the formation of liquid crystal phase was identified by the observation of optical polarization microscopy (OPM). The results showed that the content of liquid crystal in composite membrane must be more than 30% (wt) in order to form liquid crystal phase on the composite membrane surface. The blood-compatibility of the composite membranes was assessed from SEM observation of the platelet's adhesion to membrane's surface, blood clotting time and haemolysis ratio. The observation of platelet's adhesion showed that the platelets gathered together on the pure polyurethane films, but the amount of platelets which were adherent on the surface covered by the liquid crystal phase was fewer than that of pure polyurethane film when platelet-rich plasma was allowed to be in contact with the membranes for 1 h at room temperature. The determination of blood clotting time and haemolysis ratio showed that these polyurethane/liquid crystal composite membranes, in which the content of liquid crystal was more than 30% (wt), appear to be beneficial in improving the blood compatibility and reducing the thrombogenicity.