Abstract
Blood, sweat and tears: a review of non-invasive DNA sampling
Highlights
Peer Community Journal is a member of the Centre Mersenne for Open Scientific Publishing http:// www.centre-mersenne.org/
When DNA analysis is required for purposes such as sexing, kinship and differentiation between cryptic species prior to experimentation with the same individuals, the DNA sampling procedure could bias the results of the subsequent experiment
To draw a comprehensive picture of the current use of these methods, we conducted a systematic review of the recent literature (2013-2018) and discuss what non-invasive DNA sampling is used for as well as issues relating to the misuse of the term
Summary
We conducted a keyword-based search on the Web Of Science core collection using the keywords DNA and non-invasive or DNA and noninvasive, as both spellings were originally proposed and are in common use (Taberlet et al, 1999; Taberlet & Luikart, 1999). A total of 380 papers were retained in our final dataset (see list in Supplementary Table 1) This dataset may not be exhaustive; it is taken to be representative of the current literature on non-invasive DNA sampling. The results from these searches were used as non-exhaustive but comparable numeric estimates only, and were not further curated. Where multiple methods were used in the same study, these were classified as compliant with the definition by Taberlet et al (1999) only if all the methods used were compliant or if invasive sampling methods were clearly identified from non-invasive ones The latter required screening of the whole paper.
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