Blood Pressure in Wild‐type and Ts65Dn Mice at 6 Months of Age

Abstract

Down syndrome (Ds) results from triplication of the 21st chromosome, and is estimated to occur 1 in every 700 births in the United States. Many people with Ds exhibit cardiovascular challenges including pulmonary hypertension and lower resting blood pressure. We tested the hypothesis that Ts65Dn mice, a model of Ds, would exhibit lower blood pressure compared to wild‐type mice. The CODA volume‐pressure tail cuff system by Kent Scientific (Torrington, CT) was used to quantify systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) in 6–month wild‐type (n=9) and Ts65Dn (n=7) male mice. Two trial days were administered for each mouse that included 15 inflation/deflation cycles of the cuff on each day. The trial was considered successful if ≥7 of the 15 cycles were accepted by the software. The mean of the 7–15 cycles was calculated for each mouse (DBP, SBP and MAP separated). If the 2nd trial mean differed by >10 mmHg for SBP the mouse was excluded from the analysis. Data are shown as wild‐type vs. Ts65Dn; MEAN ± SD; * = p<0.05. Ts65Dn mice displayed a reduced SBP (104 ± 9 vs. *96 ± 4 mmHg). DBP (77 ± 9 vs. 69 ± 5 mmHg) and MAP (85 ± 9 vs. 78 ± 4 mmHg) were not different in this early analysis. These data suggest that Ts65Dn mice may be an appropriate model to use in understanding cardiovascular function in Ds. Further investigation is warranted to confirm these findings across the lifespan of wild‐type and Ts65Dn mice.Support or Funding InformationFunded by 1 R15 HD076379‐01A1 (LRD), HKB supported by the Ignite fellowship and Le Moyne College Student Research Committee, CNR supported by 1 R15 HD076379‐01A1S1.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.