Blood parameters and reproductive function of highly productive cows using an organic immunomodulatory drug

Abstract

The aim of the study was to correct the morphofunctional status of highly productive cows before calving by using an organic immunomodulatory drug. Four groups of Holstein cows (production > 8500 kg per lactation) were formed (n=10 in each group). The immunomodulatory drug contains formic aldehyde 0.07-0.24%, sodium chlorine 0.90-0.95, distilled water. The drug was injected intramuscularly at 25-30 days before calving with an interval of 7 days, three times and 8-10 hours after calving, once (experimental group-1 – 4.0 ml, experimental group-2 - 6.0 ml, experimental group-3 - 8.0 ml). The 4th group was a control. Blood samples were taken 25-30 days before calving, 5 days before calving and 5 days after parturition. Using an immunomodulatory drug in a dose of 6.0 ml 5 days before calving, changes occur in the blood of the animal. In the blood serum of cows, the increased level of Ca by 0.5 mmol/l, P – by 0.22 mmol/l, alkaline reserve – by 4.03 vol%CO2, glucose – by 0.80 mmol/l, total protein – by 9.01 g/l, alpha-globulins – by 2.98%, Ig A – by 36.9%, Ig M – by 15.73%, Ig G – by 50.02%, reduced ratio of AlT and AsT enzymes by 17.04 and 15.74 units./l (up to the reference level), beta-globulins – by 3.44%. This contributed to a reduction in complications of calving and postpartum period by 40.0%, uterine involution by 19.04 days compared to a dose of 4 ml. The difference in criteria that characterize the morphofunctional state when using 8 ml and 6 ml is insignificant. Results of the research are statistically significant (P<0.01). Using an immunomodulatory drug in a dose of 6 ml, the cellular composition, morphobiochemical parameters of the blood are improved. The appearance of obstetric and gynecological pathologies is reduced by 40%, the retention of plaсеnta by 30%, the duration of uterine involution by 19.04 days, the period of effective insemination by 33.56 days. The fertilization of cows in the first hunt is increased by 30% than in the control group.