Abstract
The blood O2 dissociation curve was determined with a microphotometric reaction apparatus in two frog species,Rana catesbeiana andRana brevipoda, and formulated in terms of a modified Hill equation. The O2 dissociation curve was in reasonable agreement with determinations of O2 content, O2 capacity,\(P_{O_2 } \) and pH (or\(P_{CO_2 } \)) in blood samples taken from various blood vessels and heart cavities. The blood O2 affinity inRana catesbeiana (mean body weight 230 g, kept at 25°C) was low:P50=42 Torr (at 25°C and at ventricular blood pH=7.79). The blood of the smaller frog,Rana brevipoda (mean body weight 16 g), had even lower O2 affinity,P50 being 52 Torr (at 25°C, pH=7.72). The decrease ofP50 with increasing body mass, resulting from comparison of these two frog species, is in accordance with literature data on mammals and birds.
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