Abstract
Oxygen dissociation curves were determined for winter flounder (Pseudopleuronectes americanus) blood at 5, 10, and 15 C, and [Formula: see text], 8, and 24 mm Hg. Half-saturation tensions (P50’s) and blood oxygen capacities were measured and showed significant Bohr and Root effects. Calculations of Hill’s constant, n, implied no heme-heme interaction in the flounder hemoglobin. The apparent heat of oxygenation, ΔH, was calculated to be smaller than that in most teleostean hemoglobins. This small temperature effect, together with the modest slope of the hyperbolic oxygen dissociation curves, indicate a capacity to live in waters exhibiting a fairly broad range of temperatures and dissolved oxygen concentrations.
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