Abstract
Blood levels of ACTH in individual rats 3 and 10 days after bilateral adrenalectomy have been determined by the assay method of Lipscomb and Nelson, using the adrenal venous corticosteroid response of the 2-hr hypophysectomized rat. Three days after adrenalectomy ACTH levels in blood were equivalent to 0.08 μ0.03 mU ACTH/ml; 10 days after adrenalectomy levels were 0.27μ0.05 mU/ml. Pre-rinsing syringes with an acid-gelatin solution prior to collection of donor blood did not significantly alter blood levels of ACTH. The ability to measure ACTH levels in the blood of individual rats 3 and 10 days after adrenalectomy supports the previous finding of an increased sensitivity of ACTH assay with the use of the 2-hr hypophysectomized rat and measurement of adrenal venous effluent corticosterone levels. The data suggest the feasibility of determining ACTH levels in blood of individual intact rats following conditions of stress. (Endocrinology 75: 483, 1964)
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
