Abstract

Abstract The aim of this study was to track the changes in blood parameters of mid-lactation multiparous Polish Holstein-Friesian cows in response to a diet supplemented with a mixture of fish-soybean oils blend and tannin-containing lingonberry shrub (Vaccinium vitis idaea; VVI) extract. Twelve lactating cows were randomly assigned to a crossover design of two treatments (6 cows per treatment) which consisted of a control diet containing no supplement (CON) and CON supplemented with a mixture of 99 g of VVI leaves extract and 660 g of blended fish-soybean oils (MIX) daily. The obtained results showed a significant increase in plasma glucose level, as well as C18:1t11 and n-3 fatty acids proportion. A significant decrease was also observed in insulin concentration, triglyceride and C18:0 proportion. Generally, the saturated fatty acid proportion decreased while the unsaturated fatty acid significantly increased with the MIX diet. In conclusion, using supplements of a mixture of VVI extract and fish-soybean oils blend modulated the unsaturated fatty acid proportion in blood, without affecting the dairy cows’ blood parameters which were all within the normal ranges.

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