Abstract
The problem of blood-grouping Peruvian mummies involves the theories on the origin of ABO polymorphism and current hypotheses concerning the peopling of the New World. Some attempts to perform these determinations were made by several research-workers. Owing to different results, especially for what concerns the presence or absence of A and B antigens, some of the results seemed neither reproductive nor reliable and suggested the hypothesis for the presence of contaminating substances. Since a purification method suitable for mummified material had been set-up in our laboratory and applied with successful results to Egyptian specimens, we thought it worthwhile to try its application to ancient Peruvian material. A sample of cancellous bone was ground-up, washed with pure melted phenol and with ethanol and extracted by 5% E.D.T.A. The extract was filtered through Sephadex G 25 columns and tested with H.I. reaction using anti-A and anti-B human sera and anti-H lectin prepared, purified and controlled for its specificity in our Laboratory. Grouping with this technique 33 Inca or pre-Inca Peruvian specimens, mostly coming from Ancon and Cuzco, 28 group O were found, while 5 samples were not diagnosed.
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