Abstract
Laser scanning multi-photon microscopy (MPM) makes it possible to obtain detailed, three-dimensional images of tissue microvascular geometry. When combined with mathematical models of network blood flow and mass transport, this data will be extremely useful in estimating local oxygen delivery capabilities and correlating them with direct measures of tissue oxygenation. The objectives of this study were to reconstruct 3D microvascular networks imaged with MPM, and perform theoretical calculations of blood flow in the reconstructed networks. An intravital 3D angiogram of a complete microvascular unit in the mouse hindlimb, including feeding arterioles, capillary segments and collecting venules, was used as the basis for this study. Algorithms for skeletonizing the microvascular network and determining network connectivity and vessel diameters were implemented. The reconstructed network and a previously developed blood flow model were used to calculate steady-state hemodynamics in the network. In the future, these results will be refined using experimental measures of 3D network hemodynamics. Financial Support: HSFC, MSFHR, CIHR/HSFC IMPACT Fellowships (RMB).
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