Blood Contact Properties of Surface Immobilized C60

Abstract

The coagulation time assay employed herein measured the propensity of different surface chemistries to contact activate the intrinsic pathway of blood coagulation. By varying the number of identically prepared disks used in a coagulation assay, we can systematically vary the amount of surface area and thus study the dependence of coagulation time on both surface chemistry and surface area. The type of data that results is a plot of coagulation time versus surface area. Comparison of such raw data for different surface chemistry permits the development of structure-reactivity relationships that connect surface chemistry, energy, and the behavior of blood at interfaces. In this communication, we compare results for C[sub 60] monolayers to methyl-terminated SAMs and clean glass. In this report, an immobilized monolayer of fullerenes has been shown to be slightly activating in plasma coagulation (relative to methyl-terminated SAMs). 11 refs., 1 fig.