Blood concentrations of remifentanil during and after infusion in horses anesthetized with isoflurane and dexmedetomidine

Abstract

ObjectiveTo determine blood remifentanil concentration in isoflurane-anesthetized horses during and after a 1h remifentanil and dexmedetomidine infusion. Study designProspective study. AnimalsSix adult mixed breed horses with (mean±SD) bodyweight of 507±61kg and 14±4years of age. MethodsFollowing sedation with xylazine IV, anesthesia was induced with ketamine IV mixed with diazepam IV. Anesthesia was maintained with isoflurane in oxygen. After 52±7min for instrumentation, dexmedetomidine (0.25μgkg−1 followed by 1.0μg−1kg−1h−1) and remifentanil infusions (6μgkg−1h−1) were administered for a minimum of 60min and horses recovered from anesthesia. Drug infusions were administered into the left jugular vein. Blood was sampled (4mL) from the right jugular vein at predefined intervals before and during administration of remifentanil infusion. Following catheter flush, blood was sampled from the left jugular vein after the infusion was terminated while the horse was recovering from anesthesia. Blood was placed into tubes containing sodium heparin with citric acid, flash frozen in liquid nitrogen, and stored at −80°C until analysis. Blood remifentanil concentration was measured using high performance liquid chromatography and tandem mass spectrometry. ResultsMean peak remifentanil concentration was 7.14ηgmL−1 at 50min after start of infusion. Mean volume of distribution was 268±40mLkg−1 and mean half-life was 12.8min. Blood concentration decreased to 1ηgmL−1 27min after termination of infusion.Limit of quantification was 0.2ηgmL−1.