Blood boron concentrations in pregnant rats fed boric acid throughout gestation

Abstract

Timed-mated Sprague-Dawley rats (28 to 32/group) were exposed to boric acid (BA) in the diet from Gestational Day (GD) 0 to 20. Dietary concentrations of added BA (0%, 0.025%, 0.050%, 0.075%, 0.100%, or 0.200%) yielded average daily intakes equivalent to 0, 3, 6,10, 13, or 25 mg boron/kg body weight/d. Dams and their fetuses were evaluated for evidence of maternal or developmental toxicity, as reported previously. At termination on GD 20, maternal whole blood was collected in heparinized Vacutainer ® tubes, stored frozen (−20°C), and subsequently prepared by a high-temperature alkaline ashing procedure for analysis of boron by inductively coupled plasma optical emission spectrometry. Increasing dietary concentrations of BA were positively associated with whole blood boron concentrations in confirmed pregnant rats, specifically 0.229 ±0.143, 0.564 ± 0.211, 0.975 ± 0.261, 1.27 ± 0.298, 1.53 ± 0.546, or 2.82 ± 0.987 μg boron/g whole blood (mean ±SD) for the control through high-dose groups. Maternal blood boron concentrations were positively correlated with indices of maternal dietary intake of boron and with embryo/fetal toxicity observed at 0.100% and 0.200% BA in the diet reported previously. Thus, blood boron concentrations of 1.27 ± 0.298 and 1.53 ± 0.546 μg boron/g were associated with the no-observed-adverse-effect level (10 mg boron/kg/d) and lowest-observedadverse-effect level (13 mg boron/kg/d) for developmental toxicity reported previously.