Blood biochemical parameters in transfer factor donor cows depending on sensitization scheme

Abstract

Research and development of means for effective prevention and treatment of diseases in animals are one of the priorities for modern veterinary science. Means based on the transfer factor are quite promising to solve these problems. One of the stages of obtaining a qualitative transfer factor specific to a particular disease is the sensitization of the body of donor animals. The purpose of this work was to investigate the blood biochemical parameters of donor cows after sensitization according to different schemes. The experiments were performed on cows of the Ukrainian black-spotted dairy breed, aged 4–5 years. Sensitization of pregnant cows was performed 1–1.5 months before calving with a concentrated formol-alum vaccine against salmonellosis of calves manufactured by the Kherson Biofactory. The vaccine was administered to the animals of the first experimental group one month before calving, one-time in a dose of 10 ml. Animals of the second experimental group 1.5 months before calving were two-time vaccine administered with an interval between injections of 10 days in doses of 10 and 15 ml. Studies have shown that in donor cows, which were two-time vaccine administered, there was an increase in hemoglobin content by 13% (P<0.05). There was also a decrease in glucose and creatinine content by 13–28% (P<0.05–0.01) in the blood serum of pregnant cows, which did not depend on the sensitization scheme, and a tendency to a decrease in total protein content. Regardless of the sensitization scheme of cows, an increase in serum aminotransferase activity was observed by 1.3–1.5 times (P<0.05–0.001), and if alanine aminotransferase activity increased mainly with a single injection of the vaccine, then aspartate aminotransferase activity was more intensively increased after a two-time vaccine administration. There was a slight decrease in сalcium (by 5–9%) and phosphorus (by 2–3%) content and an increase in potassium content (by 2–5%) in the blood serum of pregnant cows two weeks after vaccine administration regardless of the sensitization scheme.