Abstract
BackgroundHemorrhagic shock remains a major cause of mortality and morbidity on the battle field. Appropriate use of blood‐based biomarkers is clinically important to manage patients and predict death versus survival in the early stage of hemorrhagic shock. In continuation of our previous study, blood‐based early biomarkers were tested in 94 rats undergoing hemorrhagic shock.MethodsSprague Dawley rats (both genders) were anesthetized with either intraperitoneal ketamine and xylazine or with Isoflurane. Rats were intubated and ventilated with room air. After heparinization, hemorrhagic shock was induced by withdrawing blood to a fixed mean blood pressure of 30 or 40 mmHg for 30 minutes, 35 minutes, 45 minutes, or 60 minutes and then the shed blood was reinfused. Arterial blood samples were collected 1–3 hours after resuscitation with shed blood. The rats were allowed to survive for 6 weeks.ResultsThe pH, bicarbonate (HCO3), oxygen saturation (SO2%), base excess and calcium levels were significantly lower in rats that died (n=63) versus survivors (n=31). Rats that died had significantly elevated anion gap, potassium, sodium and chloride levels compared to rats that survived (Table). PO2, PCO2, hematocrit, hemoglobin, glucose and lactate levels are comparable between rats that died versus survived.ConclusionsThe low pH, HCO3, SO2%, base excess and calcium coupled with high anion gap and high potassium, sodium and chloride levels may help predict long term prognosis in hemorrhagic shock.Support or Funding InformationThis work was supported by the Office of the Assistant Secretary of Defense for Health Affairs, through the Peer Reviewed Medical Research Program under Award NO. W81XWH‐16‐1‐0606. Opinions, interpretations, conclusions and recommendations are those of the author and are not necessarily endorsed by Department of Defense.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
