Abstract
The effect of one intraperitoneal injection of 1.33 ml of CCl4 per kg of fish was studied. Two experimental series were performed and studied for 10 days (with diluent) and 24 h (pure CCl4) periods. LDH, GOT, GPT, GR, GDH, CPK, G‐6‐Pase, and AlkPase were studied. The activity of all enzymes in blood increased: LDH (four times the control), GOT (two times), GPT (three times); they reached a maximal activity 12 h after injection of diluted CCl4. The levels of some enzymes were also examined in the liver. With pure CCl4, maximal enzyme activity in blood occurred earlier (6 h). A 6 to 10 times increase was observed for GOT, GPT, LDH, GR, and GDH. Histopathological observations were correlated with these enzymes studies.An Aeromonas disease characterized by the destruction of the dermis, the exposure of the muscle, and by the presence of numerous petechiae in the liver enabled us to examine the relationships between naturally induced tissue damage and enzyme levels in blood. The levels of seven blood enzymes were determined and the most significant modifications were observed for LDH and CPK. which increased their concentration from 3 to 7 times respectively. A pyruvate saturation test demonstrated that LDH was probably from liver as it was observed after CCl4 poisoning. The contribution of such biochemical studies in fish research is evaluated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have