Blocking abdominal lymphatic flow attenuates acute hemorrhagic necrotizing pancreatitis -associated lung injury in rats

He Peng,Guo Yun-Zhen,Li Yan,Wang Zhi-Fen,Chen Li-Ping,Jin Su-Mei

doi:10.1186/1476-9255-10-9

Abstract

BackgroundAcute hemorrhagic necrotizing pancreatitis (AHNP) is a severe acute inflammatory of pancreas that can lead to extrapancreatic organ disfunction. The lung and intestine is the most common involved organs, and abdominal lymphatic flow may contribute to AHNP- associated organ injury. In this study, we investigated the impact of thoracic duct ligation and drainage on lung and intestine injury in rats with AHNP.MethodsThirty-two male Wistar rats were randomly divided into 4 groups: sham operation group, AHNP group, AHNP + ligation group and AHNP + drainage group. Rat AHNP model was induced by retrograde injection of 3.5% sodium deoxycholate into the biliopancreatic duct, and the sham operation group was injected only with saline. In AHNP + ligation group and AHNP + drainage group, thoracic duct was ligated or drainaged before model induction. At 6 h after model induction, the bronchoalveolar lavage fluid (BALF) were collected for determination of tumor necrosis factor-alpha (TNF-alpha), and the tissues of lung, intestine and pancrease were harvested individually for pathohistological evaluation and the myeloperoxidase (MPO) activity determination. In addition, the activity of serum amylase and diamine oxidase (DAO) was determined in each group.ResultsThe pathohistological damage and MPO of lung, intestine and pancrease, TNF-alpha of BALF, serum amylase and DAO were all increased in AHNP group compared to those in sham operation group (P < 0.05). In AHNP + ligation group, the pathohistological damage and MPO of lung and TNF-alpha of BALF were reduced, but the pathohistological damage and MPO of intestine and pancrease were increased compared with AHNP group (P < 0.05), however the activity of serum amylase and DAO was no changed. In AHNP + drainage group, the pathohistological damage and MPO of lung, intestine and pancrease, TNF-alpha of BALF, serum amylase and DAO were all reduced compared to those in AHNP group (P < 0.05).ConclusionsOur finding suggest that thoracic duct ligation can reduce neutrophil infiltration and TNF-alpha release and then attenuates lung injury in rats with AHNP, but aggravates the injury of intestine and pancrease. While thoracic duct drainage attenuates the injury of lung, intestine and pancreaseat the same time in rats with AHNP.

Highlights

Acute hemorrhagic necrotizing pancreatitis (AHNP) is a severe acute inflammatory of pancreas that can lead to extrapancreatic organ disfunction
Acute hemorrhagic necrotizing pancreatitis (AHNP) is a severe acute inflammatory disease of the pancrease that can lead to intestinal barrier failure and subsequent translocation of bacterial and endotoxin, which promotes the development of multiple organ dysfunction
The effect of blocking abdominal lymphatic flow on histopathological injury of lung, intestine and pancrease in AHNP rats Lung Diffuse pulmonary capillary dilatation and congestion, intense alveolar septum swelling and breakage, part of alveolar structural damage and heavy infiltration of inflammatory cells mostly neutrophils were found in the lung tissue of AHNP group (Figure 1B)

Summary

Introduction

Acute hemorrhagic necrotizing pancreatitis (AHNP) is a severe acute inflammatory of pancreas that can lead to extrapancreatic organ disfunction. The lung and intestine is the most common involved organs, and abdominal lymphatic flow may contribute to AHNP- associated organ injury. We investigated the impact of thoracic duct ligation and drainage on lung and intestine injury in rats with AHNP. Acute hemorrhagic necrotizing pancreatitis (AHNP) is a severe acute inflammatory disease of the pancrease that can lead to intestinal barrier failure and subsequent translocation of bacterial and endotoxin, which promotes the development of multiple organ dysfunction. The lung is the most common involved extra-gut organ both in AHNP patients and animal model [1,2]. In AHNP animals, when thoracic lymphatic duct was ligated, the morbidity of rat was decreased in one study [6]. How lymph-flow interruption effects AHNP remains a question

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Results

Conclusion