Blockage of KCa3.1 and Kv1.3 channels of the B lymphocyte decreases the inflammatory monocyte chemotaxis

Abstract

ObjectiveTo investigate the effects of Ca2+ activated potassium channel KCa3.1 and voltage-gated potassium channel Kv1.3 of B lymphocyte on inflammatory monocytes chemotaxis and the potential mechanisms. Materials and methodsThanswell test was used to detect the inflammatory monocyte (Ly-6Chi) chemotaxis caused by the B lymphocyte. Enzyme-linked immunosorbent assay (ELISA) was applied to detecting the C-C motif ligand 7 (CCL7) in cultured media. Cell counting kit-8 (CCK) was used to detect the proliferation of B lymphocytes after activation and blockage of both KCa3.1 and Kv1.3 channels. Western blot was used to detect the expression of phosphorylated extracellular signal-regulated kinase (P-ERK) of the B lymphocytes. ResultsWhen activated, B lymphocytes significantly proliferated. After application of KCa3.1 channel-specific inhibitor TRAM-34 and potent Kv1.3 channel inhibitor ShK, both B lymphocytes proliferation and Ly-6Chi monocyte chemotaxis were significantly inhibited. The expression of chemotaxis related factor CCL7 decreased remarkably. ConclusionThe opening of KCa3.1 and Kv1.3 channels promote B lymphocyte activation, proliferation and Ly-6Chi monocyte chemotaxis. The increase of CCL7 secretion by B lymphocyte may explain the pro migration effects.