Blockade of IL-11 Trans-Signaling or JAK2/STAT3 Signaling Ameliorates the Profibrotic Effect of IL-11

Abstract

ABSTRACT Objectives Systemic sclerosis (SSc) is a rare rheumatic disease characterized by vascular damage, dysregulated immune response, and fibrosis. Interleukin−11 (IL−11) is upregulated in SSc. This study aimed to investigate the pathological and therapeutic role of the IL−11 trans-signaling pathway in SSc. Methods Plasma IL−11 level was evaluated in 32 patients with SSc and 15 healthy controls, while the expression levels of ADAM10, ADAM17, IL−11, IL−11 Rα, or IL−11 co-stained with CD3 or CD163 in the skin of SSc patients and healthy controls were analyzed. Fibroblasts were treated with IL−11 and ionomycin to evaluate the profibrotic effect of IL−11 trans-signaling pathway. TJ301 (sgp130Fc) and WP1066 (a JAK2/STAT3 inhibitor) intervention groups were set up to investigate the antifibrotic effect of targeting IL−11. Results Levels of plasma IL−11 were extremely low in most SSc patients and healthy controls. In contrast, levels of IL−11, IL−11 Rα, and ADAM10, but not ADAM17, were significantly elevated in the skin of SSc patients. Moreover, the numbers of IL−11+ CD3+ cells and IL−11+ CD163+ cells were increased in the skin of SSc patients. Besides, IL−11 and ADAM10 were also elevated in the skin and pulmonary of bleomycin-induced SSc mouse. Fibroblasts co-stimulated with IL−11 and ionomycin showed increased expression of COL3 and phosphorylation of STAT3, which could be inhibited by TJ301 or WP1066. TJ301 also ameliorated skin and lung fibrosis in BLM-induced SSc mouse. Conclusions IL−11 induces fibrosis in SSc by regulating the trans-signaling pathway. Blockage of sgp130Fc or inhibition of the JAK2/STAT3 pathway could ameliorate the profibrotic effect of IL−11.