Block copolymer-derived recessed nanodisk-array electrodes for electrochemical detection of β-lactam antibiotics

Abstract

Antimicrobial resistance (AMR) is one of the major socio-economic factors contributing to public health. β-lactams are most commonly prescribed drugs for variety of bacterial infections. Frequent use of antibiotics leads to AMR in humans and animals. The present work is focused on developing an electro-immunosensor to control and regulate the excessive use of antibiotics in animal-based food products. An amphiphilic block co-polymer poly(ethylene oxide-block-methyl methacrylate)(PEO-b-PMMA) was used to fabricate recessed nano-disk array electrode (RNE) and immobilized with Pen-Ab and Cef-Ab antibodies. The Limit of detection (LOD) of RNE working electrode was found to be 14.8 pM for penicillin and 13.8 pM for cefalexin with good selectivity in presence of non-specific antibiotics. Fabricated RNE electrode could detect trace amounts of spiked antigen in real samples of milk, egg and meat extract. Further, mesoporous thin film and microarrays can eventually be used to develop point-of-care diagnosis of antibiotics in animal-based food products.