Abstract
Abstract Apparatus for foam separation, and its application to the isolation of surface active components of rumen liquor, are described in detail. Preliminary results demonstrate that foam fractions of rumen liquor drawn prior to bloating, and non-bloating feeds of red clover, show differences in concentrations of non-acid lipid, fatty acid, and carbohydrate components that are not reflected in the rumen liquor samples themselves. Foam separation has been used to isolate saponins from rumen liquor Yellow bubbles containing more than 30% lipid were found present in the leading fraction of foam separated from rumen liquor drawn prior to feeding. There were more present when bloat resulted from the following feed than when no bloat occurred. Yellow bubbles were also observed in foam obtained from bloated cattle.
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