Abstract
The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the development of the Blastocystis serological test. Crude protein was extracted from the yeast of Blastocystis sp, then inoculated into rabbits to produce the antibody of crude protein. The serum of rabbits would be collected before and after immunization to compare the antibody titer. The profile of crude protein was analyzed using SDS-Page. The rabbit serum was analyzed using ELISA and Western Blot. The SDS-Page result showed bands in 100 kDa, 90 kDa, 70 kDa, 60 kDa, 58 kDa, 50 kDa, 40 kDa, 35 kDa, 30 kDa and 27 kDa. The ELISA assay showed that there was an increase in antibody titer of crude protein after immunization. Western Blot showed that three proteins (30 kDa, 40 kDa and 50 kDa) having immunogenicity characteristic. It is concluded that protein 30 kDa, 40 kDa and 50 kDa prepared from the crude protein of Blastocystis sp. can be used for developing a serologic test for Blastocystis infection. Keywords: Blastocystis sp, Crude Protein, Polyclonal Antibody .
Highlights
Blastocystis is an intestinal parasite in humans and a wide range of animals
The infection of Blastocystis sp generally occurs through the fecal-oral route from drinking water (Skotarczak, 2018) and food contamination (Ahmed et al, 2018)
The PCR result confirmed that the parasite used in this study is Blastocystis sp
Summary
Blastocystis is an intestinal parasite in humans and a wide range of animals (cattle, pigs, horses, birds, amphibians and reptiles). The human who often makes contact with Blastocystisinfected animals is prone to get infected by Blastocystis. The infection of Blastocystis sp generally occurs through the fecal-oral route from drinking water (Skotarczak, 2018) and food contamination (Ahmed et al, 2018). It is often found in Printed ISSN 2502-9568; Electronic ISSN 2622-8734 immunocompromised patients such as people with HIV/AIDS (Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome) or cancer. Infection was found in people with close animal contact (Wawrzyniak et al, 2013) A higher risk of Blastocystis sp. infection was found in people with close animal contact (Wawrzyniak et al, 2013)
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