Abstract
The aim of the IVF cycle is to choose the best embryo with the highest potential to the implantation. The blastocyst formation rate (BFR) is one of the main criteria to choose the embryo for the transfer. Male factor influences the level of BFR. 49 infertile couples with the male factor of infertility were undergoing the treatment of infertility by ART using the ICSI procedure. The age of the men in chose group was 37.22±4.46 years old.The correlation between value of BFR with the level of aneuploidies in sperm was investigated. Examination of aneuploidies for chromosomes 13, 16, 18, 21, X, Y in spermatozoa nuclei was done using the method of FISH with Vysis-Abbott DNA probes (USA). The obtained data were documented with fluorescent microscope Nikon Eclipse 80i and cytogenetic program Lucia FISH (Laboratory Imaging LTD, Czech Republic). BFR and the quality of the formatted blastocysts were evaluated on the fifth day of embryo development. There was no significant correlation between BFR and the quality of blastocysts and the level of aneuploidies in sperm for chromosomes 16, 18, 21, X and Y. But the significant negative correlation between the level of the sperm aneuploidy for chromosome 13 and the rate of formation of the high quality blastocysts is proved (rs=-0.57, Rcritical=0.37, N=46, p<0.01 - for the level of high quality blastocysts). There is no correlation between BFR and the age of the patients (rs = 0.01, Rcritical=0.29, N=49, p>0.05 - for total BFR; rs = 0.15, Rcritical=0.29, N=49, p>0.05 - for the level of high quality blastocysts). The BFR correlates with the level of aneuploidies in sperm. BFR of the high quality embryos is significantly decreases with the increasing of the level of sperm aneuploidy for chromosome 13 (p<0,01). Total BFR and the quality of formatted blastocysts do not depend on male age. As the blastocysts’ morphology doesn’t correlate with the level of sperm aneuploidies for chromosomes 16, 18, 21, X and Y the PGD should be recommended for the patients with the high level of aneuploidies in sperm to choose the embryo with the normal karyotype for embryo transfer.Tabled 1Correlation between the BFR and the level of aneuploidies in sperm.ChromosomesBFR, %R sR criticalNpBFR, total1348.98 ± 15.20-0.210.2946p>0.05BFR, total1650.54 ± 14.95-0.140.3238p>0.05BFR, total1849.01 ± 15.39-0.110.2949p>0.05BFR, total2149.01 ± 15.390.230.2949p>0.05BFR, totalX, Y49.01 ± 15.39-0.060.2949p>0.05BFR, high quality blastocysts1331.77 ± 11.72-0.570.3746p<0.01BFR, high quality blastocysts1631.46 ± 12.02-0.150.3238p>0.05BFR, high quality blastocysts1831.59 ± 12.13-0.050.2949p>0.05BFR, high quality blastocysts2131.59 ± 12.130.240.2949p>0.05BFR, high quality blastocystsX, Y31.59 ± 12.130.080.2949p>0.05 Open table in a new tab
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