Black soybean may be effective to recover the impaired insulin signaling in high cholesterol diet induced non‐alcoholic fatty liver disease mice model

Abstract

NAFLD is strongly associated with insulin resistance and is defined by accumulation of liver fat more than 5% per liver weight. In this study, we investigated the effects of low and high concentrations of black soybean on the insulin signaling of mice fed high‐cholesterol diets. Mice were randomly allocated into 5 groups that were fed different diets: the normal cholesterol diets (NCD); high‐cholesterol diets (HCD); and HCD with 1%, and 4% black soybean powder (1B‐HCD, and 4B‐HCD, respectively). Blood glucose and insulin were significantly lower in the black soybean‐supplemented groups (129.2mg/dl, 1.76μIU/ml; 113.7mg/dl, 2.29μ IU/ml) than that in the HCD group (163.2mg/dl, 3.07μIU/ml). The serum HOMA‐IR and free fatty acid concentration were significantly higher in the HCD group than in the NCD, and it was decreased by black soybean supplementation. We measured adiponectin as it is critical adipokine involved in insulin resistance in NAFLD. The adiponectin was significantly lower in the HCD group than that in the NCD and significantly increased with black soybean supplementation. These results suggest that the black soybean restores the fasting glucose via adiponectin secretion.Grant Funding Source: ASN