Abstract

Biochar (BC) has rich nutrient content and soil-improving properties. BC has recently been used as a fertilizer or soil amendment material. However, studies in which BC is applied with sulfur (S) are limited. In this study, effects of BC obtained from olive pulp and S separately and in combination with phosphorus (P) in soil with clay loam and high pH effects on enzyme activities -important for soil health- (acid phosphatase, alkaline phosphatase, β-Glucosidase and Dehydrogenase) have been examined. Different BC (0, 0.75%, 1.5%) and S (0, 800 mg kg-1) doses have been applied to the soil and incubated under controlled conditions for 45 days according to the factorial trial plan. According to the results of this study, BC and S interaction on electrical conductivity (EC), Total P and β-Glucosidase enzyme activity were found to be significant. BC and S application increased the total phosphorus amount, while both BC and S applications increased the available amount of P. However, BC and S resulted in an increase in soil EC value and S application decreased soil pH value. In addition, the amount of soil organic matter increased with the increase in the dose of BC. Strikingly while BC and S applications did not affect enzyme activity acid phosphatase, alkaline phosphatase and dehydrogenase, β-glucosidase enzyme activity was decreased in S application without BC application. This preliminary study shows that BC and S applications are required to be studied at appropriate field conditions and at appropriate doses.

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