Bixin and Annatto seeds Extract maintains ADM levels to Sustain Pregnancy

Abstract

Bixa orellana (family Bixaceae) is a neotropical rapid growing perennial tree of immense agro-industrial value since its seeds have a high carotenoid content, chiefly bixin. It has been utilized since pre-colonial times as a culinary colorant and spice, and for healing purposes. It is at present used as a natural pigment in the food, in pharmaceutical and cosmetic industries, and it is commercially acknowledged as annatto. We have investigated the antioxidant property of bixin which have the capability to reduce blood pressure during pregnancy and maintain ADM levels to sustain pregnancy. A diet rich in bixin and iron content sustain pregnancy in rats. The present study aimed to evaluate the effect of the treatment with aqueous extract of annatto seed and isolated bixin on the rats. Similarly to evaluate the importance of adrenomedullin hormones, we have injected rats with ADM22-52 antagonist. Experimental group of Rats especially Group-I and II on the gestational day 2 were implanted subcutaneously with osmotic (Alzet) minipumps delivering 125 and 250 µg rat/day/of AM22–52 and were killed on the gestational day 9. Group-III experimental rats was administered orally with bixin during early pregnancy and group-IV was control. Our results demonstrated that rats administered orally with isolated bixin compound, sustained pregnancy and ADM antagonist injected group showed fetal resorption. We have ascertained the hypothesis that ADM, a multiregulatory ubiquitous peptide hormone, works as a trophoblast pro-invasion factor, as a growing body of evidence which clearly signifies that bixin enhances ADM levels which in turn helps to sustain healthy pregnancy to full term baby.