Abstract
Photo 1. Cerastoderma edule (Linnaeus, 1758). (A) Emerged animals at low tide, (B) submerged animals with foot (F) and inhalant (IS) and exhalent (ES) siphons deployed. Animals in (A) and (B) are in their 3rd year (two annual growth rings are visible, corresponding to periostracal thickening) and thus both sexually mature. (C) Oocytes (OC) double-stained with Neutral Red vital stain and Trypan Blue mortal stain. Left, normal oocyte, right, dead oocyte. TB, undissolved Trypan Blue stain. (D) Oocytes stained with Neutral Red only; normal oocytes (NO) stain red, while dead oocytes do not stain. The TB stain proved effective, but cumbersome and costly to use in routine studies, while the more convenient and cost-efficient Neutral Red stain alone provided the same information. Photo credit: D. Chérel. These photographs illustrate the article “Evolutionary and ecological insights from vital staining of bivalve oocytes: A Red Queen at the sweepstakes?” by Peter G. Beninger, Daphné Chérel, Gaël Le Pennec, and Sandra E. Shumway published in Ecosphere. https://doi.org/10.1002/ecs2.4047
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