Bituminous coal and sodium hydroxide-pretreated seawater stimulates Spirulina (Arthrospira) maxima growth with decreased production costs

Abstract

Spirulina is a photosynthetic cyanobacterium rich in protein, polyunsaturated fatty acids, minerals and vitamins. Spirulina spp. are produced in fresh water, although freshwater shortages and the high-cost of feed stock carbon limit Spirulina production. Thus, use of seawater with low-cost carbon should be evaluated. Conversely, Ca 2 + and Mg 2 + of natural seawater (NSW) cause milky turbidity and nutrient precipitation when used in Spirulina culture medium. Here, NSW was pretreated with bituminous coal and NaOH to sediment Ca 2 + and Mg 2 + and obtain total organic carbon (TOC)-enriched pretreated seawater supernatant (PSWS), which was used to formulate Spirulina maxima culture medium. Pretreatment of NSW with bituminous coal and NaOH showed Ca 2 + and Mg 2 + precipitation rates of 32.52% and 99.91%, respectively, while TOC increased 6.97-fold in PSWS compared to NSW. The PSWS was 90–93% of NSW by volume, excluding sediment. The dry biomass production was 1.55 and 1.57 g L − 1 and the chlorophyll a content was 13.75 and 17.90 mg L − 1 in SOT and formulated AKSM-2-1 media, respectively. The maximum specific growth rate (μ max day − 1 ) and biomass productivity of S. maxima were 0.283 and 0.284 day − 1 and 1.97 and 2.00 g L − 1 day − 1 in SOT and formulated media, respectively. The biochemical composition and phycocyanin content were similar in the biomass of both media. Biomass production costs were ca. threefold lower in formulated medium than SOT medium. Therefore, S. maxima can be produced at lower costs using NSW following our method. • Pretreatment of natural seawater (NSW) with bituminous coal and NaOH made adequately sediment of Ca 2 + and Mg 2 + . • Total organic carbon increased ~ 7 times in pretreated seawater supernatant (PSWS) than NSW. • Use of PSWS resolved K 2 HPO 4 solubility problem in seawater Spirulina maxima culture medium. • Biomass production and biomass quality were same in formulated and SOT media. • Biomass production cost ~ 3 times lower in formulated medium than that of conventional medium.