Bisphosphorylated fructose-modified magnetic Zr-Organic framework: A dual-hydrophilic sorbent for selective adsorption of immunoglobulin G

Abstract

Highly selective adsorption of protein species interested from complicated biological samples is an extremely important prerequisite for in-depth proteomic analysis. Herein, a novel dual-hydrophilic material is fabricated by grafting Zr-based metal-organic framework (Zr-MOF) onto the surface of magnetic microspheres, and subsequent modifying with fructose-1,6-bisphosphate (FBP) molecules by taking advantage of multivalent binding between the phosphate backbone of FBP and Zr centers. Owing to the favorable dual-hydrophilicity derived from the abundant amino groups in Zr-MOF and the hydroxyl groups in FBP molecules, the obtained magnetic microspheres (mMOF-FBP) provide high adsorption efficiency, favorable selectivity and excellent capturing capacity (555.6 mg g−1) towards immunoglobulin G (IgG). The captured IgG could be collected from the mMOF-FBP by using 0.5% (m/v) NH3·H2O as elution reagent with a recovery of 88.4%. The dual-hydrophilic microspheres demonstrate their practicability by selective isolation of IgG from human serum.