Abstract
Bisphenol B (BPB), a substitute of bisphenol A (BPA), is widely used in the polycarbonate plastic and resins production. However, BPB proved to be not a safe alternative to BPA, and as an endocrine disruptor, it can harm the health of humans and animals. In the present study, we explored the effects of BPB on mouse oocyte meiotic maturation in vitro. We found that 150 μM of BPB significantly compromised the first polar body extrusion (PBE) and disrupted the cell cycle progression with meiotic arrest. The spindle assembly and chromosome alignment were disordered after BPB exposure, which was further demonstrated by the aberrant localization of p-MAPK. Also, BPB exposure increased the acetylation levels of α-tubulin. As a result, the spindle assemble checkpoint (SAC) was continuously provoked, contributing to meiotic arrest. We further demonstrated that BPB severely induced DNA damage, but the ROS and ATP production were not altered. Furthermore, the epigenetic modifications were changed after BPB exposure, as indicated by increased K3K9me3 and H3K27me3 levels. Besides, the pattern of estrogen receptor α (ERα) dynamics was disrupted with a mass gathering on the spindle in BPB-exposed oocytes. Our collective results indicated that exposure to BPB compromised meiotic maturation and damaged oocyte quality by affecting spindle assembly and chromosome alignment, acetylation of α-tubulin, DNA damage, epigenetic modifications, and ERα dynamics in mouse oocytes.
Highlights
Endocrine disrupting chemicals (EDCs), a group of exogenous substances or compound mixtures, can interfere with hormone action in the body and disrupt endocrine function that harms human and animal health (WHO/IPCS, 2002)
The objective of this study was to evaluate the effects of bisphenol A (BPA) on mouse oocyte maturation and its related mechanisms in vitro, and by evaluating the ratio of polar body extrusion (PBE), ROS levels, DNA damage, spindle morphology, chromosome alignment and segregation, ER, and epigenetic modifications
Results showed that Bisphenol B (BPB) exposure compromised the oocyte meiotic maturation (Figure 1A)
Summary
Endocrine disrupting chemicals (EDCs), a group of exogenous substances or compound mixtures, can interfere with hormone action in the body and disrupt endocrine function that harms human and animal health (WHO/IPCS, 2002). Bisphenol A (BPA), a common manufacturing chemical in polycarbonate plastics is being widely used in industrial production, is one of the most abundant EDCs (Usman and Ahmad, 2016). BPA has been attracting increasing attentions, and many researchers are digging to explore how the endocrine system disrupted by bisphenol B (BPB) (Weatherly and Gosse, 2017; Ma et al, 2019a; Yang et al, 2019). BPA exposure decreases testosterone levels, affects sperm production and quality, and increases sperm DNA damage (Wisniewski et al, 2015; Lombó et al, 2019). Due to its reproductive toxicity and endocrine disrupting properties, the use of BPA is restricted
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
