Bisphenol A suppresses interferon-gamma production and related biochemical pathways in human PBMC

Abstract

Event Abstract Back to Event Bisphenol A suppresses interferon-gamma production and related biochemical pathways in human PBMC Emanuel Raggl1, Simon Geisler1, Johanna M. Gostner1, Dietmar Fuchs1* and Harald Schennach1 1 Innsbruck Medical University, Austria Bisphenol A (BPA) is one of the most widely distributed chemicals. Humans are exposed to it mainly via polycarbonate plastics that are used for food packaging and plastic bottles and contain BPA as a softener. There is accumulating evidence of a carcinogenic and allergy-promoting effect of BPA. In vitro we investigated the impact of BPA on freshly isolated human peripheral blood mononuclear cells (PBMC) from healthy donors. PBMC were incubated with increasing doses of BPA, and after 30 min either left unstimulated or stimulated with the mitogen phytohemagglutinin (PHA) and cell viability was controlled. After 48 h, formation of interferon-gamma (IFN-gamma), macrophage product neopterin as well as tryptophan breakdown by indoleamine 2,3-dioxygenase (IDO), expressed as the kynurenine to tryptophan ratio (Kyn/Trp), were monitored. All these immunobiochemical pathways are characteristic for cell-mediated (= Th1-type) immune response. Upon PHA stimulation, neopterin production and tryptophan breakdown increased significantly compared to unstimulated cells. BPA exerted a dose-dependent suppressive effect in unstimulated and in PHA-stimulated PBMC at 12.5 - 200 µM concentrations. Due to the cross-regulation of Th1- and Th2-type immunity, the suppressive effects of BPA on Th1-type immunity could relate to the allergy-promoting properties of the compound. Moreover, the inhibition of IFN-gamma production and its related antiproliferative effects could be of relevance for the tumor-promoting effect of this chemical. On the other side, the antioxidant compound could also have beneficial effects, possibly delaying or even preventing Th1-type immunopathologies. References Zaknun D, Schroecksnadel S, Kurz K, Fuchs D. Potential role of antioxidant food supplements, preservatives and colorants in the pathogenesis of allergy and asthma. Int Arch Allergy Immunol 2012;157:113-124 Gostner JM, Becker K, Fuchs D, Sucher R. Redox regulation of the immune response. Redox Report 2013;18: 88-94 Gostner J, Ciardi C, Becker K, Fuchs D, Sucher R. Immunoregulatory impact of food antioxidants. Curr Pharm Des (in press) Keywords: bisphenol A, Interferon-gamma, Neopterin, indoleaminedioxygenase, PBMC Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Raggl E, Geisler S, Gostner JM, Fuchs D and Schennach H (2013). Bisphenol A suppresses interferon-gamma production and related biochemical pathways in human PBMC. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00665 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Dietmar Fuchs, Innsbruck Medical University, Innsbruck, Austria, dietmar.Fuchs@i-med.ac.at Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Emanuel Raggl Simon Geisler Johanna M Gostner Dietmar Fuchs Harald Schennach Google Emanuel Raggl Simon Geisler Johanna M Gostner Dietmar Fuchs Harald Schennach Google Scholar Emanuel Raggl Simon Geisler Johanna M Gostner Dietmar Fuchs Harald Schennach PubMed Emanuel Raggl Simon Geisler Johanna M Gostner Dietmar Fuchs Harald Schennach Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.