Bisphenol a Hormonal Disrupture and Preventive Effect of Rose Water and Clove Oil

Abstract

Bisphenol A (BPA) which considered synthetic estrogen that is an essential component of many plastic industries. This research was done to see the impacts of exposure of BPA on reproductive organs and hormonal levels in male and female albino Sprague-Dawley rats. The protective effect of rose water and clove oil on BPA was investigated. Ninety rats were divided into 18 groups, 9 groups of males and they are like for females. Rats were exposed to different oral gavage route 3 times a week by doses of BPA (20 µg, 20 mg, 200 mg) /kg b.wt for 6 weeks and BPA was solubilized in corn oil. BPA induced a significant decrease in total and free testosterone in male rats, in contrast to a significant increase in thyroid-stimulating hormone (TSH), progesterone, estrogen (E2), and prolactin (PRL), while a decrease in Follicle-stimulating hormone (FSH) compared to control groups. Histopathological examination revealed that rosewater and clove oil reduced testes and ovary damages induced by BPA. Rosewater and clove oil components were scanned using GC/MS, which showed that rosewater and clove oil contains phenols, flavonoids, and these inevitably confirm that a prominent role in preventing the damage during treatment. Results indicated that the used doses of BPA disrupted the sex hormone levels in both male and female rats caused reproductive impaired. The chemical and histopathological analysis results indicated that clove oil and rose water improved the adverse effect of BPA. Rosewater and clove oil improved the changes which were stimulated by BPA.