Abstract
Bisphenol A (BPA) is a xenoestrogen chemical commonly used to manufacture polycarbonate plastics and epoxy resin and might affect various human organs. However, the cellular effects of BPA on the eyes have not been widely investigated. This study aimed to investigate the cellular cytotoxicity by BPA exposure on human retinoblastoma cells. BPA did not show cytotoxic effects, such as apoptosis, alterations to cell viability and cell cycle regulation. Comparative analysis of the transcriptome and proteome profiles were investigated after long-term exposure of Y79 cells to low doses of BPA. Transcriptome analysis using RNA-seq revealed that mRNA expression of the post-transcriptional regulation-associated gene sets was significantly upregulated in the BPA-treated group. Cell cycle regulation-associated gene sets were significantly downregulated by exposure to BPA. Interestingly, RNA-seq analysis at the transcript level indicated that alternative splicing events, particularly retained introns, were noticeably altered by low-dose BPA treatment. Additionally, proteome profiling using MALDI-TOF-MS identified a total of nine differentially expressed proteins. These results suggest that alternative splicing events and altered gene/protein expression patterns are critical phenomena affected by long-term low-dose BPA exposure. This represents a novel marker for the detection of various diseases associated with environmental pollutants such as BPA.
Highlights
Bisphenol A (BPA) is widely used in the manufacturing of plastics, epoxy resin liners of canned foods, beverage containers, dental materials, and medical devices
To investigate the cellular physiological effects of low-dose BPA exposure on retinal cells, Y79 cells were treated with various BPA concentrations for 24 and 48 h
Cells were treated with BPA (20–1000 μM)
Summary
Bisphenol A (BPA) is widely used in the manufacturing of plastics, epoxy resin liners of canned foods, beverage containers, dental materials, and medical devices. BPA is a xenoestrogen chemical with estrogen-like activity associated with adverse effects on the human endocrine systems. It is among the most debated examples of endocrine-disrupting chemicals (EDCs) [5]. Several studies have investigated the adverse effects of BPA on the central nervous system owing to its lipophilic properties, which can lead to its accumulation in the brain [10,11]. Intrauterine BPA exposure can lead to sex-specific epigenetic alterations in the brain, even at low doses [12]. Based on this accumulating evidence, many countries have banned BPA use. There are increasing public concerns about its harmful effect, as humans are constantly exposed to such environmental pollutes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
