Bisdemethoxycurcumin attenuates gastric adenocarcinoma growth by inducing mitochondrial dysfunction.

Changjiang Luo,Zhixing Du,Zhongxue Fu,Xing Wei,Gang Chen

doi:10.3892/ol.2014.2685

Abstract

Bisdemethoxycurcumin (BDMC) is a demethoxy derivative of curcumin. In this study, a human gastric adenocarcinoma xenograft model was generated in vivo using nude mice and BDMC was observed to suppress the growth and activity of tumors, in addition to improving the physical and mental capacity of the mice. An increased number of apoptotic cells, decreased ratio of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein and increased caspase-3 expression was also observed following treatment with BDMC, indicating that BDMC may promote apoptosis in tumors via mitochondrial modulation. The growth of SGC 7901 gastric cancer cells was inhibited and arrested at G1 phase. Specific indicators of mitochondrial dysfunction, a reduction in adenosine triphosphate generation, the inner mitochondrial membrane potential, augmentation of reactive oxygen species production and cytochrome c were also detected in the mitochondria following treatment with BDMC. These results indicate that BDMC attenuates gastric adenocarcinoma growth by inducing mitochondrial dysfunction.

Highlights

Of novel drug molecules with low toxicity for the treatment of gastric adenocarcinoma is required
The current study investigated the effect of BDMC on the growth inhibition of gastric adenocarcinoma in vivo and in vitro
BDMC, together with curcumin and demethoxycurcumin, are the three predominant active compounds derived from the turmeric root

Summary

Introduction

Of novel drug molecules with low toxicity for the treatment of gastric adenocarcinoma is required. Previous studies have focused on the anticancer effects of natural products, which are considered to exhibit reduced toxicity. Bisdemethoxycurcumin (BDMC) is a demethoxy derivative of curcumin, which is a natural substance found in the turmeric root. BDMC is significantly more stable than curcumin in physiological media. Whether it arrests the cell cycle of gastric adenocarcinoma and attenuates gastric adenocarcinoma growth remains unclear. The current study investigated the effect of BDMC on the growth inhibition of gastric adenocarcinoma in vivo and in vitro. As mitochondria are critical for the growth of tumors, the mitochondrial function of gastric adenocarcinoma cells following BDMC treatment was analyzed

Methods

Results

Conclusion