BIRC5 Expression is Regulated in Uterine Epithelium During the Estrous Cycle.

Minha Cho,Jihyun Lee,Kwonho Hong,Sohyeon Moon,Haeun Park,Sujin Lee,Eun Mi Chang,Youngsok Choi,Chankyu Park,Keun-Hong Park,Ok-Hee Lee

doi:10.3390/genes11030282

Minha Cho, Jihyun Lee + Show 9 more

Open Access

https://doi.org/10.3390/genes11030282

Copy DOI

Journal: Genes	Publication Date: Mar 6, 2020
Citations: 3	License type: CC BY 4.0

Affiliation: Konkuk University, CHA Gangnam Medical Center, CHA University

Abstract

Baculoviral inhibitor of apoptosis repeat-containing 5 (Birc5), also known as survivin, is a member of the inhibitor of apoptosis (IAP) family of proteins and regulates the size of tissues through cell division control. The uterus is the most dynamically sized organ among tissues during the estrous cycle. Although Birc5 is expressed in some terminally differentiated cells, the regulation of its expression in the uterus remains unknown. We investigated the regulation of Birc5 expression in the mouse uterus. RT-PCR analysis showed that Birc5 was expressed in various tissues, including the uterus; the expression level of Birc5 was significantly higher at the diestrus stage. Immunohistochemistry and Western blotting analysis revealed that Birc5 was more active in luminal and glandular epithelium than in endometrial stroma. In ovariectomized mice, Birc5 expression in the uterus was gradually increased by estrogen treatment; however, progesterone injection decreased its expression. Estrogen-induced Birc5 expression was blocked by treatment with estrogen receptor antagonist, ICI 182, 780 and progesterone-reduced Birc5 expression was inhibited by the progesterone receptor antagonist RU486. These results suggest that Birc5 expression is dynamically regulated by a combination of estrogen and progesterone via their receptor-mediated signaling.

Highlights

The uterus is a female reproductive organ that plays an important role in several pregnancy processes such fertilization, implantation, and embryonic development [1]
In order to confirm the expression of Baculoviral inhibitor of apoptosis repeat-containing 5 (Birc5) mRNA, we performed Reverse Transcription PCR (RT-PCR) analysis using total
We presented the regulation of Birc5 expression in the uterus

Summary

Introduction

The uterus is a female reproductive organ that plays an important role in several pregnancy processes such fertilization, implantation, and embryonic development [1]. The endometrium of the uterus is periodically regulated according to the estrous cycle. The menstrual cycle has an interval of. It corresponds to the estrous cycle of 4–5 days in the rodents (reviewed in [2]). The estrous cycle consists of four stages viz. The precise regulation of the estrous cycle within ≈4–5 days is very important for a successful pregnancy. The uterine endometrium is dynamically regulated by the ovarian sex hormones estrogen and progesterone. The endometrium undergoes proliferation, differentiation, and shedding (humans and primates) or apoptosis (rodent) in response to hormones during the cycle [2]

Methods

Results

Conclusion