Abstract
A 3-month outbreak of invasive group A Streptococcus disease at an eldercare facility, in which 5 persons died, was biphasic. Although targeted chemoprophylaxis contained the initial outbreak, a second phase of the outbreak occurred after infection control processes ended. To retrospectively investigate the genomic epidemiology of the biphasic outbreak, we used whole-genome sequencing and multiple bioinformatics approaches. Analysis of isolates from the outbreak and isolates prospectively collected during the outbreak response indicated a single S. pyogenes emm81 clone among residents and staff members. Outbreak isolates differed from nonoutbreak emm81 isolates by harboring an integrative conjugative genomic element that contained the macrolide resistance determinant erm(TR). This study shows how retrospective high-resolution genomic investigations identified rapid spread of a closed-facilty clonal outbreak that was controlled, but not readily cleared, by infection control management procedures.
Highlights
A 3-month outbreak of invasive group A Streptococcus disease at an eldercare facility, in which 5 persons died, was biphasic
We screened core genomes for mutations within the key group A Streptococcus (GAS) regulatory genes covR/S, ropB, mga, which had previously been linked to increased virulence among GAS isolates [1]; we found no differential mutations between outbreak and nonoutbreak isolates
Through our clinical and genomic epidemiologic analyses, we determined that a fatal GAS outbreak in an eldercare facility was associated with a single emm81 GAS clone that was resistant to erythromycin and exhibited inducible clindamycin resistance. emm81 GAS is one of the most common M-types that causes invasive disease in New Zealand [3]
Summary
A 3-month outbreak of invasive group A Streptococcus disease at an eldercare facility, in which 5 persons died, was biphasic. To retrospectively investigate the genomic epidemiology of the biphasic outbreak, we used whole-genome sequencing and multiple bioinformatics approaches. Outbreak isolates differed from nonoutbreak emm isolates by harboring an integrative conjugative genomic element that contained the macrolide resistance determinant erm(TR). This study shows how retrospective high-resolution genomic investigations identified rapid spread of a closed-facilty clonal outbreak that was controlled, but not readily cleared, by infection control management procedures. The incidence of invasive GAS infections is highest among older adults [2,3], those living in longterm care facilities [2,4]. Outbreaks of GAS infections are often linked with distinct epidemiologic markers such as emm type [5,6]. Outbreaks of GAS infections are often linked with distinct epidemiologic markers such as emm type [5,6]. emm typing is a sequence-
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.