Abstract
Blue light regulates biological function in various cells, such as proliferation, oxidative stress, and cell death. We employed blue light illumination on human umbilical vein endothelial cells utilizing a LED device at 453 nm wavelength and revealed a novel biphasic response on human umbilical vein endothelial cells (HUVECs). The results showed that low fluence blue light irradiation promoted the fundamental cell activities, including cell viability, migration and angiogenesis by activating the angiogenic pathways such as the VEGF signaling pathway. In contrast, high fluence illumination caused the opposite effect on those activities by upregulating pro-apoptotic signaling cascades like ferroptosis, necroptosis and the p53 signaling pathways. Our results provide an underlying insight into photobiomodulation by blue light and may help to implement potential treatment strategies for treating angiogenesis-dependent diseases.
Highlights
Endothelial cells (ECs) constitute the blood vessel’s innermost [1,2,3] lining and play a critical role in maintaining the homeostasis of blood vessels
To understand the effect of blue light irradiation on human umbilical vein endothelial cells (HUVECs)’ viability, cells were treated with two intensities, 10 mW/cm2 and 20 mW/cm2, for different time durations based on the irradiation parameters used in previous studies [23,24,25]
XTT assay results show that HUVECs responded biphasically to the blue light exposure
Summary
Endothelial cells (ECs) constitute the blood vessel’s innermost [1,2,3] lining and play a critical role in maintaining the homeostasis of blood vessels. Angiogenesis, a physiological process of new vessel formation from existing vessels, depends on various functions of ECs. It is a complex multistep activity that occurs physiologically in embryonic development, organogenesis and wound healing [1]. It is a complex multistep activity that occurs physiologically in embryonic development, organogenesis and wound healing [1] It plays a role in pathological states including carcinogenesis, diabetic retinopathy and chronic polyarthritis [1]. A vascular anastomosis is formed with the newly formed vascular networks [2]. These steps are well-regulated by a multitude of cytokines, the extracellular matrix and proteolytic enzymes [2].
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