Biphasic anaphylaxis after first dose of messenger RNA coronavirus disease 2019 vaccine with positive polysorbate 80 skin testing result

Abstract

Initial reports of anaphylaxis after the first dose of the Pfizer-BioNTech (Pfizer Inc, New York, New York; BioNTech SE, Mainz, Germany) vaccine suggested a case rate of 11.1 per million doses, but subsequent reports estimate a rate of 5 per million doses.1CDC COVID-19 Response Team, Food and Drug AdministrationAllergic reactions including anaphylaxis after receipt of the first dose of Pfizer-BioNTech COVID-19 vaccine—United States, December 14-23, 2020.MMWR Morb Mortal Wkly Rep. 2021; 70: 46-51Crossref PubMed Google Scholar, 2Shimabukuro TT Cole M Su JR Reports of Anaphylaxis After Receipt of mRNA COVID-19 Vaccines in the US-December 14, 2020-January 18, 2021.JAMA. 2021; 325: 1101-1102Crossref PubMed Scopus (220) Google Scholar In a recent description of 21 anaphylaxis cases after the first dose of the Pfizer-BioNTech vaccine, there were no biphasic reactions.3Shimabukuro T Nair N. Allergic reactions including anaphylaxis after receipt of the first dose of Pfizer-BioNTech COVID-19 vaccine.JAMA. 2021; 325: 780-781Crossref PubMed Scopus (166) Google Scholar We present the case of an individual who experienced suspected biphasic anaphylaxis after the first dose of the Pfizer-BioNTech coronavirus disease 2019 (COVID-19) vaccine. A 42-year-old female nurse with depression and anxiety treated with bupropion but no previous atopic history or anaphylaxis presented for her first dose of the Pfizer-BioNTech COVID-19 vaccine at a nurse and paramedic staffed employee vaccination clinic in a major hospital. After 10 minutes from vaccination, she developed facial angioedema noted by multiple medical providers, sensation of throat closure, hoarse voice, and a pruritic rash on her neck and upper chest with no substantial injection site reaction. At the vaccination site, she was treated with oral diphenhydramine and 0.3 mg intramuscular epinephrine with symptom improvement. She was transported to the emergency department, where she had a heart rate of 108 bpm and blood pressure of 137/87 mm Hg. She was treated with 20 mg intravenous famotidine and 10 mg intravenous dexamethasone and observed for 6 hours without symptom recurrence. A tryptase drawn 90 minutes after symptom onset was elevated at 12.5 ng/mL (<11.5 ng/mL). She was discharged with an epinephrine autoinjector and initially did well, but the following evening (30 hours postvaccination), she developed recurrent tongue swelling (noted by husband), sensation of throat closing, eye puffiness, diaphoresis, and lightheadedness. She administered oral diphenhydramine and her epinephrine autoinjector with rapid improvement in her symptoms. She went back to the emergency department where providers noted resolution of tongue swelling but persistent periorbital edema. There was no rash. Her heart rate was 109 bpm, and her blood pressure was 121/74 mm Hg. She was treated with 10 mg intravenous dexamethasone, observed for 4 hours, and discharged with no subsequent symptom recurrence. She was evaluated in an allergy clinic 3 weeks after her reaction. Further history revealed previous tolerance of polyethylene glycol (PEG)-containing medications (MiraLAX [Bayer, Leverkusen, Germany]). She had no known drug allergies or dermal fillers. Because of her marked systemic reaction, she underwent expanded skin testing.4Banerji A Wichner PG Saf R et al.mRNA vaccines to prevent COVID-19 disease and reported allergic reactions: current evidence and suggested approach.J Allergy Clin Immunol Pract. 2021; 9: 1423-1437Abstract Full Text Full Text PDF PubMed Scopus (260) Google Scholar Testing results to PEG, methylprednisolone sodium, methylprednisolone acetate, and polysorbate 20 were negative. Skin testing result to triamcinolone acetonide (containing polysorbate 80) was negative with a 40 mg/mL skin prick and 1:100 and 1:10 intradermals but result to a 1:1 intradermal was positive (Table 1). Additional testing results were positive to Refresh sterile eye drops (Allergan, Inc., Dublin, Ireland), negative to Prevnar-13 (Wyeth Pharmaceuticals, Philadelphia, Pennsylvania) (both containing polysorbate 80), and negative to the Pfizer-BioNTech vaccine (Table 1). A baseline serum tryptase was persistently elevated at 12.8 ng/mL, although spot urine measurements of mast cell mediators (leukotriene E4, 2,3-dinor-11beta-prostaglandin F2 alpha, and N-methylhistamine) were normal. KIT D816V mutation testing result on peripheral blood was negative but testing result for hereditary alpha tryptasemia was positive with an extra alpha tryptase copy number. She is avoiding the second dose of the Pfizer-BioNTech vaccine until an in-office challenge is performed.Table 1Skin Testing ResultsStepsaControls done before testing include control prick (0), histamine 6 mg/mL SP (3 × 3 flare).PEG 3350ControlPolysorbate 20Polysorbate 80mRNA COVID-19 vaccineMiraLAX (170 mg/mL)Methylprednisolone acetate (40 mg/mL)Methylprednisolone sodium (40 mg/mL)Polysorbate 20 (0.5 mg/mL)Triamcinolone acetonide (40 mg/mL)Refresh eye dropsPrevnar 13 vaccinePfizer-BioNTech (lot number ER8731)Step 11:100 SP (0)1:1 SP (0)1:1 SP (0)1:1 SP (0)1:1 SP (0)1:1 SP (0)1:10 SP (0)1:1 SP (0)Step 21:10 SP (0)1:100 ID (0)1:100 ID (0)1:100 ID (0)1:10 ID (3 × 3 wheal, 7 × 7 flare)1:100 ID (0)Step 31:1 SP (0)1:10 ID (0)1:10 ID (0)1:10 ID (0 wheal, 4 × 4 flare)Step 41:1 ID (3 × 3 wheal, 8 × 8 flare)Abbreviations: COVID-19, coronavirus disease 2019; ID, intradermal; PEG, polyethylene glycol; mRNA, messenger RNA; SP, skin prick.a Controls done before testing include control prick (0), histamine 6 mg/mL SP (3 × 3 flare). Open table in a new tab Abbreviations: COVID-19, coronavirus disease 2019; ID, intradermal; PEG, polyethylene glycol; mRNA, messenger RNA; SP, skin prick. Several aspects of this case deserve discussion. Biphasic anaphylaxis is well described but uncommon, occurring in approximately 3% of adults who develop anaphylaxis.5Lee S Sadosty AT Campbell RL. Update on biphasic anaphylaxis.Curr Opin Allergy Clin Immunol. 2016; 16: 346-351Crossref PubMed Scopus (30) Google Scholar Risk factors for a biphasic reaction include previous anaphylaxis, unknown trigger, and delayed epinephrine administration, although these are variable among different study populations.5Lee S Sadosty AT Campbell RL. Update on biphasic anaphylaxis.Curr Opin Allergy Clin Immunol. 2016; 16: 346-351Crossref PubMed Scopus (30) Google Scholar In adults, the estimated median time from exposure to the biphasic reaction is 15 hours.6Ko BS Kim WY Ryoo SM et al.Biphasic reactions in patients with anaphylaxis treated with corticosteroids.Ann Allergy Asthma Immunol. 2015; 115 (312-216)Abstract Full Text Full Text PDF PubMed Scopus (25) Google Scholar Our case is unique in the fact that the patient had a biphasic reaction despite no history of allergy and prompt receipt of epinephrine during the initial reaction, although some symptoms may have been anxiety related. To the best of our knowledge, this is the first reported case of suspected biphasic anaphylaxis to the Pfizer-BioNTech vaccine. Another interesting aspect is the positive polysorbate 80 skin test result. Polysorbate 80 is a large nonionic compound that is a widely used excipient in many oral and injectable medications, including the Janssen COVID-19 vaccine (Janssen Pharmaceuticals, Beerse, Belgium), but is not present in the messenger RNA (mRNA) COVID-19 vaccines. It is cross-reactive with PEG, another excipient thought to be the leading culprit in allergic reactions to the mRNA COVID-19 vaccines. Immunoglobulin E and non–immunoglobulin E-mediated mechanisms are implicated in polysorbate and PEG reactions.7Castells MC Phillips EJ. Maintaining safety with SARS-CoV-2 vaccines.N Engl J Med. 2021; 384: 643-649Crossref PubMed Scopus (254) Google Scholar Expert opinion suggests an algorithm using nonirritating concentrations for PEG and polysorbate testing in the evaluation of patients with possible anaphylaxis to the mRNA COVID-19 vaccines.4Banerji A Wichner PG Saf R et al.mRNA vaccines to prevent COVID-19 disease and reported allergic reactions: current evidence and suggested approach.J Allergy Clin Immunol Pract. 2021; 9: 1423-1437Abstract Full Text Full Text PDF PubMed Scopus (260) Google Scholar There has only been 1 report of immediate hypersensitivity from a polysorbate 80-containing vaccine (Gardasil [Merck & Co., Kenilworth, New Jersey]) with positive results to skin prick and intradermal testing to this vaccine.8Badiu I Guena M Heffler E Rolla G. Hypersensitivity reaction to human papillomavirus vaccine due to polysorbate 80.BMJ Case Rep. 2012; 2012 (bcr0220125797)Crossref PubMed Scopus (66) Google Scholar It is plausible that our patient's reaction to the Pfizer-BioNTech vaccine was the result of PEG and polysorbate 80 cross-reactivity, which has been previously reported, albeit in patients with a positive PEG skin testing result.9Stone Jr, CA Liu Y Relling MV et al.Immediate hypersensitivity to polyethylene glycols and polysorbates: more common than we have recognized.J Allergy Clin Immunol Pract. 2019; 7: 1533-1540Abstract Full Text Full Text PDF PubMed Scopus (189) Google Scholar Our patient's negative results to PEG and methylprednisolone skin tests may suggest against cross-reactivity, although an alternative explanation is that the PEG concentration used for skin testing was too low to elicit a positive reaction. Owing to institutional restrictions, intradermal testing to the Pfizer-BioNTech vaccine was unable to be performed, which would be the next step in evaluation. In addition, use of Refresh is nonstandardized and based on expert opinion without established nonirritating concentrations. The sensitivity and specificity of skin testing to the vaccine and its components are unknown as is the mechanism of reaction. More patients will have to be evaluated to elucidate this further. A final interesting point is the patient's persistent tryptase elevation 3 weeks after her index reaction. Systemic mastocytosis and monoclonal mast cell activation syndrome were deemed less likely after her negative KIT mutation testing result. Nevertheless, genetic testing result for hereditary alpha tryptasemia was positive and may have contributed to her symptoms given its association with elevated basal serum tryptase and increased risk of severe anaphylaxis.10Lyons JJ Chovanec J O'Connel MP et al.Heritable risk for severe anaphylaxis associated with increased α-tryptase-encoding germline copy number at TPSAB1.J Allergy Clin Immunol. 2021; 147: 622-632Abstract Full Text Full Text PDF PubMed Scopus (85) Google Scholar Despite this case report, in the overwhelming majority of cases, the risk of morbidity and mortality from COVID-19 will far outweigh the risk associated with vaccination. Although more study is needed to determine the use of skin testing in the evaluation of vaccine reactions, the widespread vaccination of the global population is imperative in the control of the pandemic.