Biovolume and surface area calculations for microalgae, using realistic 3D models

Abstract

Morphology and spatial dimensions of microalgal units (cells or colonies) are among the most relevant traits of planktic algae, which have a pronounced impact on their basic functional properties, like access to nutrients or light, the velocity of sinking or tolerance to grazing. Although the shape of algae can be approximated by geometric forms and thus, their volume and surface area can be calculated, this approach cannot be validated and might have uncertainties especially in the case of complicated forms.In this study, we report on a novel approach that uses real-like 3D mesh objects to visualize microalgae and calculates their volume and surface area. Knowing these dimensions and their intraspecific variabilities, we calculated specific shape and surface area constants for more than 300 forms, covering more than two thousand taxa.Using these constants, the accurate volume and surface area can be quickly computed for each taxon and having these values, morphology-related metrics like surface area/volume ratio, the diameter of spherical equivalent can also be given quickly and accurately.Besides their practical importance, the volume and surface area constants can be considered as size-independent morphological traits that are characteristic for the microalgal shapes, and provide new possibilities of data analyses in the field of phytoplankton ecology.