Biouptake, toxicity and biotransformation of triclosan in diatom Cyclotella sp. and the influence of humic acid.

Abstract

Triclosan is one of the most frequently detected emerging contaminants in aquatic environment. In this study, we investigated the biouptake, toxicity and biotransformation of triclosan in freshwater algae Cyclotella sp. The influence of humic acid, as a representative of dissolved organic matter, was also explored. Results from this study showed that triclosan was toxic to Cyclotella sp. with 72h EC50 of 324.9μgL-1. Humic acid significantly reduced the toxicity and accumulation of triclosan in Cyclotella sp. SEM analysis showed that Cyclotella sp. were enormously damaged under 1mgL-1 triclosan exposure and repaired after the addition of 20mgL-1 humic acid. Triclosan can be significantly taken up by Cyclotella sp. The toxicity of triclosan is related to bioaccumulated triclosan as the algal cell numbers decreased when intracellular triclosan increased. A total of 11 metabolites were identified in diatom cells and degradation pathways are proposed. Hydroxylation, methylation, dechlorination, amino acids conjunction and glucuronidation contributed to the transformative reactions of triclosan in Cyclotella sp., producing biologically active products (e.g., methyl triclosan) and conjugation products (e.g., glucuronide or oxaloacetic acid conjugated triclosan), which may be included in the detoxification mechanism of triclosan.