Abstract
An up-flow immobilized cell bioreactor was developed using a microbial consortium, consisting of Bacillus sp., Alcaligenes sp. and Aeromonas sp., immobilized on refractory brick pieces as immobilization support. malachite green, a model triphenylmethane dye was decolourized by more than 93% within 48 h (operating conditions: initial dye concentration 30 mg l−1; flow rate 6 ml h−1). The analytical studies based on TLC and 1H NMR showed degradation of the aromatic rings of the malachite green into simpler metabolic intermediates.
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