Abstract

In this paper a possibility of applying anaerobic digestion for reduction and stabilization of the organic fraction of solid wastes generated during piloted spacecraft flights is discussed. The culture of Trihoderma viridae, capable of utilizing cellulose-containing substrates under mesophilic conditions, was studied. The strain was isolated from the internal environment of the ISS. The main purpose of the work was to test the ability of Trihoderma viridae culture to consume the products of anaerobic decomposition of gauze tissue under space flight conditions, i.e. substrate for the cultivation of the fungus served as liquid media, formed after the decomposition of thermophilic clostridia gauze wipes. Under conditions of space flight, the culture of Trihoderma viridae reduced the total concentration of volatile organic impurities remaining after the biodegradation of gauze from 34 mg / m3 to 5 mg / m3. The number of detectable volatile compounds decreased almost twice. The carried out researches have shown that the post-cleaning of liquid products of hydrolysis of gauze fabric with the help of cellulolytic fungi can be an effective component of utilization of cosmonaut hygiene items with the help of microbial communities in space flight conditions. Also under terrestrial conditions, the liquid products of biodegradation of plant wastes were further purified with the help of a community of aerobic bacteria. Chromatomass-spectrometric analysis showed that the consortium composed of aerobic mesophilic bacteria actively consumed products present in vegetable homogenates.

Highlights

  • Long terms of the planned space expeditions require the creation of the most closed LSS of crews in spacecraft with cyclic regeneration of substances in an artificial ecosystem [8]

  • Among the wastes that are formed in the conditions of manned space flight, a significant proportion is spent on personal hygiene

  • Biodegradation of personal care products using Clostridium thermocellum culture: In the present work, the effectiveness of the application of different bacterial strains for the destruction of gauze wipes was determined with a high content of the original ungrounded substrate

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Summary

Introduction

Long terms of the planned space expeditions require the creation of the most closed LSS of crews in spacecraft with cyclic regeneration of substances in an artificial ecosystem [8]. One of the main conditions for increasing the closure factor of LSS is the processing of waste in order to return the maximum proportion of the elements contained in them to the circulation of substances on board the MSC. Among the wastes that are formed in the conditions of manned space flight, a significant proportion is spent on personal hygiene (gauze napkins). A necessary element of the closed LSS of manned spacecraft are considered greenhouses for the reproduction of the vegetative part of the diet of cosmonauts. Used personal hygiene products and greenhouse waste accumulate will occupy significant volumes, which will lead to an increase in the equivalent weight of the system [3, 12]. In the perspective of interplanetary flights, based on the requirements of planetary quarantine, it is not possible to carry out the contaminated with microorganisms debris beyond the spacecraft

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