Abstract
In this work, incubations of testosterone, dehydroepiandrosterone and pregnenolone with Mucor hiemalis MRC 70325 have been reported. Incubation of testosterone afforded androst-4-en-3,17-dione (3%), 14α-hydroxyandrost-4-en-3,17-dione (9%), 6β-hydroxyandrost-4-en-3,17-dione (2%) and 14α,17β-dihydroxyandrost-4-en-3-one (62%). Incubation of dehy droepiandrosterone afforded 3β,17β-dihydroxyandrost-5-ene (6%) and 3β,7α-dihydroxyandrost-5-en-17-one (72%). Incubation of pregnenolone afforded 3β,14α-dihydroxypregn-5-en-7,20-dione (3%), 3β,7α-dihydroxy-pregn-5-en-20-one (64%) and 3β,7α,11α-trihydroxypregn-5-en-20-one (11%). 3β,14α-Dihydroxypregn-5-en-7,20-dione was identified as a new metabolite.
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